Our laboratory uses a number of techniques to analyse the functions of the PRESENILIN genes as well as other genes involved in Alzheimer's disease such as APP and SORL1. We are also using the latest genome engineering techiques to introduce human Alzheimer's disease mutations into our experimental model, the zebrafish. We then study these mutant zebrafish using "deep sequencing" analysis of gene expression to observe how the mutations change the molecular state of cells in their brains.